Regulatory mechanisms in the recycling phase of the spliceosome cycle: combining the human and zebrafish systems
Final Report Abstract
This project initially focussed specifically on the role of p110 and the LSm2-8 proteins in U4/U6 snRNP recycling. We initially worked on extending our findings published, but this work did not proceed far enough. Instead, a major effort of our subsequent work in this project was directed towards the goal to understand better how defects in a general splicing/recycling factor can lead to specific phenotypes, for example during human disease. Here we concentrated on the role of U1C, a specific component of the U1 snRNP, which recycles already early in spliceosome assembly, before the actual splicing catalysis takes place. As before for the U4/U6 snRNP-specific recycling factor p110, we were still particularly interested in the underlying regulatory networks, applying global approaches first and then focussing on genespecific mechanisms. We also continued to use in parallel the human and zebrafish system, with a clear focus on the mammalian system. Finally, in a recent review article we summarize important new technological advances in network analysis, applying these in the splicing field and focussing on transcriptome-wide approaches to map RNA-protein interactions.
Publications
- (2011). RNA-Seq analysis in mutant zebrafish reveals role of UIC protein in alternative splicing regulation. EMBOJ. 30, 1965-1976
Rösel, T., Hung, L.-H., Medenbach, J., Donde, K., Starke, S., Benes, V., Rätsch, G., and Bindereif, A.
