Probing membrane-peptide and membrane-protein interactions by high-field pulsed EPR spectroscopy (A02)

• Subject Area: Biophysics
• Term: from 2009 to 2020
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 55908123

With the successful establishment of a protocol to measure inter spin distances in peptides embedded in lipid membranes by means of pulsed EPR, our future goals will be to detect key parameters related to adaptation of model peptides, such as WALP and ¿-peptides, in various lipid environments with specific emphasis on the hydrophobic mismatch. Distance measurements in aligned membranes will reveal the tilt angle of peptide helices. Moreover, experiments at EPR high fields and frequency will give unique insight into the helix periodicity in peptides with different secondary structures.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 803: Functionality Controlled by Organisation in and between Membranes
• Applicant Institution: Georg-August-Universität Göttingen
• Co-Applicant Institution: Max-Planck-Institut für biophysikalische Chemie; (Karl-Friedrich-Bonhoeffer-Institut) (aufgelöst)
• Project Head: Professorin Dr. Marina Bennati