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Nitrogen deficiency and senescence in Arabidopsis and oilseed rape: Transcriptome response and the role of nitrate transporters

Fachliche Zuordnung Pflanzenphysiologie
Förderung Förderung von 2009 bis 2015
Projektkennung Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 46691270
 
Among the major field crops oilseed rape has a particularly low nitrogen use efficiency (NUE). For improving the NUE of oilseed rape it is important to advance the understanding of N-remobilization and -allocation mechanisms, to select genotypes with high NUE and to sensitively diagnose the N status of the plants. We work on two topics towards this goal:1) We aim to identify gene expression biomarkers that indicate early stages of N deficiency and senescence before phenotypic symptoms are visible. By transcriptome analysis of N deficiency-stressed plants we have identified hundreds of biomarker candidates in the first funding period. The reliability and robustness of the candidates for N deficiency and senescence initiation prognosis is currently validated and shall be challenged in various oilseed rape cultivars grown under different conditions.2) The results of the first funding period suggest that in Arabidopsis the nitrate transporters NRT1.5 and NRT1.7 are involved in signaling the N status between leaves and roots and in executing the plants' responses to N deficiency, including senescence initiation and an altered lipid/protein ratio in seeds. By overexpressing these NRT proteins in Arabidopsis and oilseed rape we want to investigate whether NUE can be improved.
DFG-Verfahren Forschungsgruppen
Beteiligte Person Dr. Christine Rausch
 
 

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