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The Role of the Nuclear Import Machinery in the Adaptation of Avian Influenza A Viruses to the Mammalian Host

Subject Area Virology
Term from 2009 to 2018
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 123002173
 
Final Report Year 2017

Final Report Abstract

Within the funding period of the Emmy Noether Program, we were able to report several achievements. First, we could show that the cellular import machinery mainly consisting of the importin-α/ß pathway plays a key role in influenza A virus avian-mammalian adaptation. Due to the sequence divergence between chicken and humans, avian influenza viruses need to adapt to the mammalian import machinery by acquiring adaptive mutations in their polymerase complex (PB2 627K/PB2 701N). The optimal polymerase-importin interplay then enables efficient nuclear access to the viral polymerase complex that in turn leads to increased replicative fitness in human cells. Second, we could show that these polymeraseimportin interactions are not only crucial for virus replication in vitro but also play a key role in influenza virus pathogenesis in mammals. Third, we could show that the nuclear import machinery also contributes to enhanced replication of other viruses, such as Ebola and MERS-CoV viruses. These findings suggest that common molecular pathways exist, albeit the detailed mechanisms by which the respective viruses utilize the nuclear import machinery seem to be different and need further elucidation. In summary, our findings improve our current understanding of the molecular basis of influenza virus interspecies transmission and provide a basis for the development of novel and potentially broad-spectrum antiviral therapies in future.

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