Detailseite
Projekt
Structural characterization of EXCRT-III assemblies
Antragsteller
Professor Dr. Winfried Weissenhorn
Fachliche Zuordnung
Virologie
Förderung
Förderung von 2005 bis 2013
Projektkennung
Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 13122596
Enveloped viruses such as HIV and Ebola assemble at and pinch off from cellular membranes as newly formed infectious particles. This process is largely mediated by the retroviral polyprotein Gag and the filovirus matrix protein VP40, which contain late domain sequence motifs that help to recruit cellular factors that normally act at endosomal membranes in the sorting of ubiquitylated cargo into vesicles that bud into endosomes giving rise to multi-vesicular bodies. The latter process is morphologically similar to enveloped virus budding and the interference with the protein machinery identified in multivesicular body formation leads to a defect in the pinching off of retroviral particles. Our goal is to understand the structural requirements of a late step in budding, which involves CHMP family protein assembly. We will use X-ray crystallography and biochemical approaches to analyze the structure of central CHMP family members, to study their activation and assembly mechanism, to elucidate their membrane binding properties and to obtain atomic details of their interactions with regulatory partners involved in the budding process.
DFG-Verfahren
Schwerpunktprogramme
Internationaler Bezug
Frankreich
