Struktur-Funktionsbeziehungen im funktionellen Komplex der membranständigen neuronalen Guanylatzyklase aus Photorezeptoren

• Antragstellerinnen / Antragsteller: Professor Dr. Alfred Blume; Professor Dr. Daniel Huster; Professorin Dr. Andrea Sinz
• Fachliche Zuordnung: Biophysik
• Förderung: Förderung von 2009 bis 2015
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 131296762

• Erstellungsjahr: 2015

Zusammenfassung der Projektergebnisse

In the course of this project, we were able to derive first structural models of myristoylated GCAP-2 and a GCAP-2/GC peptide complex confirming the postulated binding site between ROS-GC and GCAP-2. Also, the first structural model was presented for the GCAP-2 homodimer. Chemical cross-linking using stable isotope (15N) labeled protein was found to be indispensable for discriminating between intra- and intermolecular cross-links, i.e., within one monomer or between different monomers.

Projektbezogene Publikationen (Auswahl)

• The interaction of lipid modified pseudopeptides with lipid membranes, Org. Biomol. Chem. 9, 6998-7006 (2011)
  Scheidt, A., Sickert, A., Meier, T., Castellucci, N., Tomasini, C., Huster, D.
  
• The Presence of Membranes or Micelles Induces Structural Changes of the Myristoylated Guanylate-Cyclase Activating Protein-2, Eur. Biophys. J. 40 565-576 (2011)
  Theisgen, S., Thomas, L., Schröder, T., Lange, C., Kovermann, M., Balbach, J., Huster, D.
  
• StavroX – A Software for Analyzing Cross-Linked Products in Protein Interaction Studies, J. Am. Soc. Mass Spectrom. 23, 76-87 (2012)
  Götze, M., Pettelkau, J., Schaks, S., Bosse, K., Ihling, C.H., Krauth, F., Fritzsche, R., Kühn, U., Sinz, A.
  
• Structural Insights into Retinal Guanylylcyclase / GCAP-2 Interaction by Cross-Linking and Mass Spectrometry, Biochemistry 51, 4932-4949 (2012)
  Pettelkau, J., Schröder, T., Ihling, C.H., Olausson, B.E., Kölbel, K., Lange, C., Sinz, A.
  
• Peptide and protein binding to lipid monolayers studied by FT-IRRA spectroscopy, Biochim. Biophys. Acta (Biomembranes) 1828, 2294-2305 (2013)
  Blume, A. and Kerth, A.
  
• Structural analysis of guanylyl cyclase-activating protein-2 (GCAP-2) homodimer by stable isotope-labeling, chemical cross-linking, and mass spectrometry, J. Am. Soc. Mass Spectrom. 24, 1969-79 (2013)
  Pettelkau, J., Thondorf, I., Theisgen, S., Lilie, H., Schröder, T., Arlt, C., Ihling, C.H., Sinz A.
  
• Accessibilities of N-terminal myristoyl chain and cysteines in guanylyl cyclase-activating protein-2 (GCAP-2) studied by covalent labeling and mass spectrometry. Rapid Commun. Mass Spectrom. 28, 835-838 (2014)
  Ihling, CH., Schröder, T., Pettelkau, J., Tischer, A., Sinz, A.
  
• Guanylate cyclase-activating protein-2 undergoes structural changes upon binding to lipid surfaces, Biochim. Biophys. Acta (Biomembranes) 1838, 2767-2777 (2014)
  Margetić, A., Nanneman, D., Meiler, J.,Huster, D., Theisgen, S.
  
• Reliable Identification of Cross-Linked Products in Protein Interaction Studies by 13C-Labeled p-Benzoylphenylalanine, J. Am. Soc. Mass Spectrom. 25, 1628-1641 (2014)
  Pettelkau, J., Ihling, C.H., Frohberg, P., van Werven, L., Jahn, O., Sinz, A.
  
• Automated Assignment of MS/MS Cleavable Cross-Links in Protein 3D-Structure Analysis, J. Am. Soc. Mass Spectrom. 26, 83-97 (2015)
  Götze, M., Pettelkau, J., Fritzsche, R., Ihling, C.H., Schäfer, M., Sinz A.