Binding and regulation of the activity of the DSL ligand Serrate by E3 ligases

• Applicant: Professor Dr. Thomas Klein
• Subject Area: General Genetics and Functional Genome Biology ; Developmental Biology ; Cell Biology
• Term: from 2009 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 143484136

• Final Report Year: 2024

Final Report Abstract

Although not all experiments could be performed, the main aims have been achieved and the result have revealed a complex function of ubi, which differs for the ligands. Moreover, our findings indicate that the bipartite mode of binding of Mib1 applies to several DSL-ligands of Drosophila and mammals and is therefore of general importance. However, the binding epitopes in the ICDs of the ligands can be different (NN-motif vs NB).

Publications

• The role of ligand endocytosis in notch signalling. Biology of the Cell, 113(10), 401-418.
  Seib, Ekaterina & Klein, Thomas
  
• The meaning of ubiquitylation of the DSL ligand Delta for the development of Drosophila. BMC Biology, 21(1).
  Troost, Tobias; Seib, Ekaterina; Airich, Alina; Vüllings, Nicole; Necakov, Aleksandar; De Renzis, Stefano & Klein, Thomas