Quantitative continuous single cell analyses by flow cytometry and video-microscopy-based cell tracking (Z01)

• Subject Area: Cardiology, Angiology
• Term: from 2010 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 75732319

This project is an extension of the competence centre for multi-parameter flow cytometry and cell sorting (FACS) and video-microscopy-based cell tracking. We will integrate single cell technologies that facilitate the investigation of the function, molecular composition and behaviour of individual cells, using flow-cytometry, single cell sequencing and sensitive protein detection methods. The combination of these technologies bridges the prospective isolation of marker-defined primary endothelial cells at functional and molecular level to unravel molecular cues governing their cell fate decisions in real time.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 834: Endothelial Signalling and Vascular Repair
• Applicant Institution: Goethe-Universität Frankfurt am Main
• Project Head: Professor Dr. Michael Rieger, since 7/2017