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Projekt Druckansicht

Application of transposon mutagenesis to identify global regulatory network in actinomycetes

Fachliche Zuordnung Stoffwechselphysiologie, Biochemie und Genetik der Mikroorganismen
Förderung Förderung von 2010 bis 2013
Projektkennung Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 161577302
 
This project aims to identify gene networks, responsible for the regulation of antibiotic biosynthesis in Streptomyces coelicolor and S. sp. Tü6071, using a random transposon mutagenesis system and fluoroscence-activated cell sorting (FACS) high-throughput screening. The objective is to generate a large repository of actinomycetes events with tagged genes and to isolate mutants with changes in the antibiotic (actinorhodin, prodigiosin and phenalinolactone) production. The changes in antibiotic production will be identified through the expression level of the reporter genes GFP and CFP fused to the different promoters from actinorhodin, prodigiosin and phenalinolactone biosynthetic clusters. The mutants will be characterized by cloning and sequencing of the transposon insertion sites from the chromosomes on the rescue plasmids. The study will lead to the better understanding of the antibiotic biosynthesis regulation and to the rational and faster developing of the antibiotic overproducers. In addition, the outcome of this project is a new platform for the identification of the regulation of the metabolic processes in actinomycetes through transposon mutagenesis, reporter gene expression and FACS high-throughput screening.
DFG-Verfahren Sachbeihilfen
 
 

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