Project Details
Projekt
Regulation of plant metabolism in response to low oxygen concentrations
Subject Area
Plant Physiology
Term
from 2009 to 2018
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 163536822
In this proposal we aim to characterize signalling components that are involved in the proactive response of plants to a moderate decrease in the oxygen concentration as it occurs under physiological conditions within their tissues. To do this, we will mainly focus on investigating signalling pathways that lead to reprogramming of gene expression in response to low oxygen. In the past years, a vast array of hypoxia induced metabolic and molecular responses are already characterised by us. Now, we want to investigate the sensing and signalling cascade that is involved in regulating the various responses. We will use RT-PCR transcription factor profiling to identify oxygen related transcription factors that are involved in regulating gene expression and adaptation to low oxygen. The selected transcription factors will be characterised in-vivo and in-vitro to reveal their physiological function and their genetic interaction to trigger transcription of oxygen sensitive promoters. Target sequences will be explored in chromosomal sections that have been identified by QTL analysis of the low-oxygen response. A luciferase reporter screen is used to find oxygen signalling mutants defective in genes that link the changes in gene expression to low oxygen.
DFG Programme
Research Grants
