Multifunctional colloidal nanoparticles for dual imaging with fluorescence, magnitic and radioactive detection

• Applicant: Professor Dr. Wolfgang Parak
• Subject Area: Biological and Biomimetic Chemistry
• Term: from 2010 to 2014
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 170468592

• Final Report Year: 2014

Final Report Abstract

Multifunctional nanoparticles, comprising an inorganic core, and organic polymer shell were synthesized. The first functionality is the functionality of the particle core. Concerning imaging the core can provide contrast for fluorescence imaging, magnetic resonance imaging, or radioactivity imaging. The second functionality is incorporated in the polymer shell. Functional molecules can comprise organic molecules like fluorophores, or chelators for metal ions. Concerning imaging therefore also the polymer shell can provide contrast for fluorescence imaging, magnetic resonance imaging, or radioactivity imaging. Due to combining 3 different cores and 3 different polymer shells all different combinations required for bimodal imaging were obtained. These particles were used for dual imaging. Hereby the goal was to visualize the fate of the nanoparticle core and the polymer shell separately, after in vitro incorporation by cells, or after in vivo administration. By providing the core and the polymer shell two different fluorescence labels, or by providing the core and the polymer shell two different radioactive labels the fate of both entities could be followed. The results indicate that the polymer shell can be partly enzymatically degraded and therefore comes partially off the particles. Likewise, also degradation of the protein corona around nanoparticles was observed. The results show, that nanoparticles are not one entity but comprise different components, such as inorganic core, organic surface coating, as the protein corona. Multifunctional labelling allows for the observation of the fate of the different components. In vitro as in vivo, the nanoparticles may not remain one entity, but due to degradation the different components may have different final bio-distribution.

Publications

• A General Synthetic Approach for Obtaining Cationic and Anionic Inorganic Nanoparticles via Encapsulation in Amphiphilic Copolymers. Small, Vol. 7. 2011, Issue 20, pp. 2929–2934.
  Geidel, C., et al.
  
• How colloidal nanoparticles could facilitate multiplexed measurements of different analytes with analyte-sensitive organic fluorophores. ACS Nano, Vol. 5. 2011, Issue 1, pp. 21-25.
  Abbasi, A.Z., et al.
  
• Multifunctional Nanoparticles for Dual Imaging. Analytical Chemistry, Volume 83. 2011, Issue 8, pp. 2877–2882.
  Z. Ali, W. J. Parak, et al.
  
• Polymer-Coated Nanoparticles: A Universal Tool for Biolabelling Experiments. Small, Vol. 7. 2011, Issue 22, pp. 3113–3127.
  Zhang, F., et al.
  
• Integration of Organic Fluorophores in the Surface of Polymer-Coated Colloidal Nanoparticles for Sensing the Local Polarity of the Environment. ChemPhysChem, Vol. 13. 2012, Issue 4, pp. 1030–1035.
  Amin, F., et al.
  
• Physicochemical properties of protein-coated gold nanoparticles in biological fluids and cells before and after proteolytic digestion. Angewandte Chemie, International Edition, Vol. 52. 2013, Issue 15, pp. 4179–4183.
  Chanana, M., et al.
  
• Europium-quantum dot nanobioconjugates as luminescent probes for time-gated biosensing. Journal of Biomedical Optics, Vol. 19. 2014, Issue 10: 101506.
  Cywiński, P.J., et al.
  
• In vivo integrity of polymer-coated gold nanoparticles. Nature Nanotechnology, Vol. 10. 2015, pp. 619–623.
  Kreyling, W., et al.