Single-molecule fluorescence spectroscopy and imaging of the structure and dynamics of macromolecular assemblies (A06)

• Subject Area: Biophysics
• Term: from 2010 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 105286809

The core goal of the project is the development of a fluorescence-optical method for three-dimensional localization and co-localization of single fluorescent molecules with ~1 nm isotropic resolution. For this purpose, we will combine two techniques: single-molecule localization based super-resolution microscopy (STORM, PALM, PAINT, MinFlux) with metal Induced Energy Transfer (MIET) imaging. We will use this method to image structural details of fluorescently labelled macromolecular complexes with nanometer resolution and high specificity, but also to resolve temporal dynamics of structural changes with a temporal resolution down to a few nanoseconds.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 860: Integrative Structural Biology of Dynamic Macromolecular Assemblies
• Applicant Institution: Georg-August-Universität Göttingen
• Project Head: Professor Dr. Jörg Enderlein