Coupled binding and folding in protein assemblies (B02)

• Subject Area: Structural Biology
• Term: from 2010 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 105286809

Intrinsically disordered proteins (IDPs) and protein regions play important roles in assembly and regulation of dynamic multiprotein complexes and liquid-liquid phase separated states. The dynamic nature of IDPs, however, poses a huge challenge for characterization of their conformations and dynamics. I propose to use NMR spectroscopy to solve the 3D structure of the inner mitochondrial Tim50IMS/Tim23IMS complex and its modulation by presequences and Tim17IMS. In addition, I plan to study the conformations and phase separations of the intrinsically disordered C-terminal region of RNA polymerase II.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 860: Integrative Structural Biology of Dynamic Macromolecular Assemblies
• Applicant Institution: Georg-August-Universität Göttingen
• Project Head: Professor Dr. Markus Zweckstetter