Project Details
Projekt Print View

Heterologous Production and Characterization of Multiheme Cytochrome c Enzymes

Subject Area Metabolism, Biochemistry and Genetics of Microorganisms
Term from 2010 to 2016
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 186439814
 
Final Report Year 2019

Final Report Abstract

Multiheme Cytochromes c are a diverse family of electron carriers and redox enzymes that play a central role in several metabolic pathways within the biogeochemical nitrogen cycles of nitrogen, sulfur and iron. Their heme cofactors pack into conserved interaction motifs that allow for strong electronic coupling and efficient electron transfer. In this project, various multiheme cytochromes c have been recombinantly produced in either Escherichia coli or Wolinella succinogenes cells using sophisticated gene expression and cytochrome c biogenesis systems. The studied cytochromes c included diheme cytochrome c peroxidases, pentaheme cytochrome c oxidoreductases of the NrfA family, octaheme cytochrome c oxidoreductases of the Hao and MccA families and the nonaheme cytochrome c OcwA involved in metal reduction by Gram-positives. The cytochromes were purified and characterized in terms of enzyme activities, substrate/product range, spectroscopic features, high-resolution crystal structures and evolutionary implications. The results contributed to our understanding of the structure/function relationships of periplasmic/extracellular cytochrome c-dependent electron transport networks that are crucial for many environmentally significant processes.

Publications

 
 

Additional Information

Textvergrößerung und Kontrastanpassung