Complexin and synaptotagmin in Ca2+-triggered exocytosis (A11)

• Subject Area: Molecular Biology and Physiology of Neurons and Glial Cells
• Term: from 2011 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 157660137

The project investigates the action of CpxII and its interplay with the major Ca2+ sensors Synaptotagmin I and VII in Ca2+-triggered exocytosis of chromaffin cells. Starting from the corresponding null mutants (single and double null mutants) and in the framework of detailed structure-function analysis, we aim to achieve this goal by using a combination of electrophysiological, biochemical, and fluorescence microscopic techniques (confocal/STED). Furthermore, we will study CpxII’s role in gliotransmission from astrocytes and will investigate its impact on integrative functions of hippocampal signaling.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 894: Ca2+-Signalling: Molecular Mechanisms and Integrative Functions
• Applicant Institution: Universität des Saarlandes
• Project Head: Professor Dr. Dieter Bruns