Project Details
Projekt Print View

Hypoxia and vasa recta function

Subject Area Anatomy and Physiology
Nephrology
Term from 2011 to 2021
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 152203169
 
Contrast media induced nephropathy (CIN) is the third most common cause of hospital-acquired AKI1. The steady increase of coronary interventions underscores the demand for an effective prevention and therapy of CIN. In accordance to the unifying hypothesis of this Research Unit, we propose that reduced blood flow and oxygen tension to the outer renal medulla is a key feature causing CIN. Juxtamedullary nephrons are responsible for the blood supply of the medulla. We, therefore, investigate the contribution of afferent (AA) and efferent arterioles (EA), and the distal tubule (Macula densa) to contrast media (CM) induced impairment of renal hemodynamics. CM may act directly on AA, EA, and tubules. Damage of endothelial and smooth muscle cells and the release of vasoactive substances from tubules may add to perturb local hemodynamics. Acute experiments will be performed in the isolated AA and EA. The double perfused juxtaglomerular apparatus will serve for investigating tubuloglomerular interactions. CM-effects on arteriolar tone and reactivity, and underlying cellular mechanisms will be investigated with special attention to ROS, NO, Ang II, and adenosine, which are crucial for renal medullary blood flow control and the tubuloglomerular feedback. Further we investigate cytotoxicity of CM on the endothelium and the protective action of adrenomedullin on endothelial cells in renal vessels.
DFG Programme Research Units
International Connection Sweden
Major Instrumentation hypoxstation
Instrumentation Group 3060 Atemgas- und Blutgas-Analysatoren
Participating Person Dr. Mattias Carlström
 
 

Additional Information

Textvergrößerung und Kontrastanpassung