Deciphering the role of H2O2-signalling originating from different cellular compartments and cell types

• Applicant: Professorin Dr. Veronica Maurino
• Subject Area: Plant Physiology
• Term: from 2011 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 198630041

• Final Report Year: 2018

Final Report Abstract

In summary, the transcriptional dynamics of the TFs after H2O2 production was assessed and the analysis of subcellular localization and potential H2O2- triggered mobility of selected TFs was initiated. We are now continuing the proposed activities of the proposal to reach the aims and produce publications. Follow-up analysis of the biological function of At3g02840 should still be performed. We showed induced resistance of GO5 against C. higginsianum, which is completely dependent on WRKY33. We now hypothesize that indole carbonyl nitriles might play a role in defense against C. higginsianum and will work further in this direction. We found that detoxification of RCS involves at least three subcellular compartments and depends on a specific GLXI isoform during germination. Furthermore, our work allow to propose that GLXI;like proteins are not active GLX. Further work aiming to understand the molecular and biological function of GLXI-like proteins is in progress. We wrote a review article on our work on damage-control systems for small molecules.

Publications

• (2015) Activation of cyclic electron flow by hydrogen peroxide in vivo. PNAS 112, 5539-5544
  Strand DD, Livingston AK, Satoh-Cruz M, Froehlich JE, Maurino VG, Kramer DM
  (See online at https://doi.org/10.1073/pnas.1418223112)
• (2017) Defense against reactive carbonyl species involves at least three subcellular compartments where individual components of the system respond to the cellular sugar status. Plant Cell 29, 3234-3254
  Schmitz J, Dittmar IC, Brockmann JD, Schmidt M, Hüdig M, Rossoni AW, Maurino VG
  (See online at https://doi.org/10.1105/tpc.17.00258)
• (2018) Biochemical control systems for small molecule damage in plants. Plant Signal. Behav. 13(5):e1477906
  Hüdig M, Schmitz J, Engqvist MKM, Maurino VG
  (See online at https://doi.org/10.1080/15592324.2018.1477906)
• (2018) Dissecting the physiological function of Glyoxalase I and Glyoxalase I-like proteins. Front. Plant Sci. 9:1618
  Schmitz J, Rossoni AW, Maurino VG
  (See online at https://doi.org/10.3389/fpls.2018.01618)
• Control systems for small molecule damage in plants, HHU Düsseldorf, PhD Thesis, 2018
  Meike Hüdig