Structure and conformational flexibility of RNA studied by EPR spectroscopy (A03)

• Subject Area: Biophysics
• Term: from 2011 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 161793742

Pulsed EPR spectroscopy with rigid spin labels will be used to study structure and conformational flexibility (dynamics) of several RNA motives. We will do this as a function of the environment (molecular crowding), the ion concentration and the changes introduced by ligand or protein binding. Additionally, we will extend the EPR methodology to experiments at room temperature and measurements in cells.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 902: Molecular Principles of RNA-based Regulation
• Applicant Institution: Goethe-Universität Frankfurt am Main
• Project Head: Professor Dr. Thomas F. Prisner