Statistical reconstruction methods for time varying nanoscale imaging problems (A06)

• Subject Area: Statistical Physics, Nonlinear Dynamics, Complex Systems, Soft and Fluid Matter, Biological Physics
• Term: from 2011 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 28586557

The aim of this project is the development of rigorous statistical analysis methods for single marker switching (SMS) nanoscopy and their experimental verification. This will be built on the one hand on the development of a statistical physical model for SMS nanoscopy and on the other hand on the development of specific alignment methods for long sequences of spatially and temporally highly resolved images, where in each image only a sparse random fragment of the sample is visible. This will pave the way to our long term goal: quantitative superresolution imaging with given statistical precision for the estimation of absolute fluorophore concentration, respectively molecule numbers, intramolecular transition rates and dark state lifetimes.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 755: Nanoscale Photonic Imaging
• Applicant Institution: Georg-August-Universität Göttingen
• Project Heads: Professor Dr. Alexander Egner; Professor Dr. Axel Munk