Entwicklung und Anwendung analytischer Methoden für die metabolische Zellanalyse unter physiologischen und pathologischen Bedingungen

• Antragstellerinnen / Antragsteller: Privatdozentin Dr. Katja Dettmer-Wilde; Professor Dr. Wolfram Gronwald; Professor Dr. Peter Oefner, seit 1/2018; Professor Dr. Rainer Spang
• Fachliche Zuordnung: Hämatologie, Onkologie
• Förderung: Förderung von 2011 bis 2020
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 190230491

• Erstellungsjahr: 2019

Zusammenfassung der Projektergebnisse

Over the entire project duration of the KFO, we have significantly extended our method portfolio to comprehensively analyze tumor metabolism and its role in immune escape and tumor progression. This includes among others the analysis of tryptophan metabolites, 2-HG, total GSH, and 13C tracer analysis by hyphenated mass spectrometry or non-uniform sampling by NMR. The analysis of metabolites was complemented by proteomics investigations implementing for example the newly available “SWATH-mass spectrometry” technique. In addition to the instrumental analysis, we optimized and expanded our data analysis strategies, e.g. data normalization, causal modelling, etc. Overall, investigators from the core project contributed with support from the KFO to 40 publications in total over the two funding periods not considering publications that are currently in the review/revision stage.

Projektbezogene Publikationen (Auswahl)

• (2012) Performance evaluation of algorithms for the classification of metabolic 1H-NMR fingerprints. Journal of Proteome Research 11:6242-6251
  Hochrein, Jochen; Klein, Matthias S.; Zacharias, Helena U.; Li, Juan; Wijffels, Gene; Schirra, Horst Joachim; Spang, Rainer; Oefner, Peter J. & Gronwald, Wolfram
  
• (2013) Diclofenac inhibits lactate formation and efficiently counteracts local immune suppression in a murine glioma model. Int. J. Cancer 132:843-853
  Chirasani, Sridhar R.; Leukel, Petra; Gottfried, Eva; Hochrein, Jochen; Stadler, Katrin; Neumann, Bernhard; Oefner, Peter J.; Gronwald, Wolfram; Bogdahn, Ulrich; Hau, Peter; Kreutz, Marina & Grauer, Oliver M.
  
• (2013) Distinct metabolic differences between various human cancer and primary cells. Electrophoresis 34(19):2836–2847
  Dettmer, Katja; Vogl, Franziska C.; Ritter, Axel P.; Zhu, Wentao; Nürnberger, Nadine; Kreutz, Marina; Oefner, Peter J.; Gronwald, Wolfram & Gottfried, Eva
  
• (2013) New aspects of an old drug - diclofenac targets MYC and glucose metabolism in tumor cells. PLoS One 8(7):e66987
  Gottfried, Eva; Lang, Sven A.; Renner, Kathrin; Bosserhoff, Anja; Gronwald, Wolfram; Rehli, Michael; Einhell, Sabine; Gedig, Isabel; Singer, Katrin; Seilbeck, Anton; Mackensen, Andreas; Grauer, Oliver; Hau, Peter; Dettmer, Katja; Andreesen, Reinhard; Oefner, Peter J. & Kreutz, Marina
  
• (2014) CLL-cells induce IDOhi CD14+HLA-DRlo myeloid-derived suppressor cells that inhibit T-cell responses and promote TRegs. Blood 124(5):750–760
  Jitschin, Regina; Braun, Martina; Büttner, Maike; Dettmer-Wilde, Katja; Bricks, Juliane; Berger, Jana; Eckart, Michael J.; Krause, Stefan W.; Oefner, Peter J.; Le Blanc, Katarina; Mackensen, Andreas & Mougiakakos, Dimitrios
  
• (2014) Collagen XVI induces expression of MMP9 via modulation of AP-1 transcription factors and facilitates invasion of oral squamous cell carcinoma. PLoS ONE 9(1):e86777
  Bedal, Konstanze B.; Grässel, Susanne; Oefner, Peter J.; Reinders, Joerg; Reichert, Torsten E. & Bauer, Richard
  
• (2014) Continuous water infusion enhances atmospheric pressure chemical ionization of methyl chloroformate derivatives in gas chromatography coupled to time-of-flight mass spectrometry-based metabolomics. Analytical Chemistry 86(18):9186-9195
  Wachsmuth, Christian J.; Dettmer, Katja; Lang, Sven A.; Mycielska, Maria E. & Oefner, Peter J.
  
• (2015) Causal Modeling of Cancer-Stromal Communication Identifies PAPPA as a Novel Stroma-Secreted Factor Activating NFκB Signaling in Hepatocellular Carcinoma. PLoS Computational Biology 11(5):e1004293
  Engelmann, Julia C.; Amann, Thomas; Ott-Rötzer, Birgitta; Nützel, Margit; Reinders, Yvonne; Reinders, Jörg; Thasler, Wolfgang E.; Kristl, Theresa; Teufel, Andreas; Huber, Christian G.; Oefner, Peter J.; Spang, Rainer & Hellerbrand, Claus
  
• (2015) Data Normalization of 1H-NMR Metabolite Fingerprinting Datasets in the Presence of Unbalanced Metabolite Regulation. Journal of Proteome Research 14:2897-2905
  Hochrein, Jochen; Zacharias, Helena U.; Taruttis, Franziska; Samol, Claudia; Engelmann, Julia C.; Spang, Rainer; Oefner, Peter J. & Gronwald, Wolfram
  
• (2015) Enhanced metabolite profiling using a redesigned atmospheric pressure chemical ionization source for gas chromatography coupled to highresolution time-of-flight mass spectrometry. Analytical Bioanalytical Chemistry 407(22): 6669-6680
  Wachsmuth, Christian J.; Hahn, Thomas A.; Oefner, Peter J. & Dettmer, Katja
  
• (2016) LDHA-associated lactic acid production blunts tumor immunosurveillance by T and NK cells. Cell Metabolism 24(5):657–671
  Brand, Almut; Singer, Katrin; Koehl, Gudrun E.; Kolitzus, Marlene; Schoenhammer, Gabriele; Thiel, Annette; Matos, Carina; Bruss, Christina; Klobuch, Sebastian; Peter, Katrin; Kastenberger, Michael; Bogdan, Christian; Schleicher, Ulrike; Mackensen, Andreas; Ullrich, Evelyn; Fichtner-Feigl, Stefan; Kesselring, Rebecca; Mack, Matthias; Ritter, Uwe; ... & Kreutz, Marina
  
• (2016) Metformin inhibits proliferation and migration of glioblastoma cells independently of TGF-β2. Cell Cycle 15(13):1755-66
  Seliger, Corinna; Meyer, Anne-Louise; Renner, Kathrin; Leidgens, Verena; Moeckel, Sylvia; Jachnik, Birgit; Dettmer, Katja; Tischler, Ulrike; Gerthofer, Valeria; Rauer, Lisa; Uhl, Martin; Proescholdt, Martin; Bogdahn, Ulrich; Riemenschneider, Markus J.; Oefner, Peter J.; Kreutz, Marina; Vollmann-Zwerenz, Arabel & Hau, Peter
  
• (2016) Suppressive effects of tumor cell-derived 5'-deoxy-5'-methylthioadenosine on human T cells. Oncoimmunology 5(8):e1184802
  Henrich, Frederik C.; Singer, Katrin; Poller, Kerstin; Bernhardt, Luise; Strobl, Carolin D.; Limm, Katharina; Ritter, Axel P.; Gottfried, Eva; Völkl, Simon; Jacobs, Benedikt; Peter, Katrin; Mougiakakos, Dimitrios; Dettmer, Katja; Oefner, Peter J.; Bosserhoff, Anja-Katrin; Kreutz, Marina P.; Aigner, Michael & Mackensen, Andreas
  
• (2017) Biological and clinical significance of tryptophan-catabolizing enzymes in cutaneous T-cell lymphomas. Oncoimmunology 6(3):e1273310
  Maliniemi, Pilvi; Laukkanen, Kirsi; Väkevä, Liisa; Dettmer, Katja; Lipsanen, Tuomas; Jeskanen, Leila; Bessede, Alban; Oefner, Peter J.; Kadin, Marshall E. & Ranki, Annamari
  
• (2017) Microbiota disruption induced by early use of broad-spectrum antibiotics is an independent risk factor of outcome after allogeneic stem cell transplantation. Journal of the American Society for Blood and Marrow Transplantation 23(5):845–852
  Weber, Daniela; Jenq, Robert R.; Peled, Jonathan U.; Taur, Ying; Hiergeist, Andreas; Koestler, Josef; Dettmer, Katja; Weber, Markus; Wolff, Daniel; Hahn, Joachim; Pamer, Eric G.; Herr, Wolfgang; Gessner, André; Oefner, Peter J.; van den Brink, Marcel R.M. & Holler, Ernst
  
• (2017) Srebp-controlled glucose metabolism is essential for NK cell functional responses. Nature Immunology 18(11):1197–1206
  Assmann, Nadine; O'brien, Katie L.; Donnelly, Raymond P.; Dyck, Lydia; Zaiatz-Bittencourt, Vanessa; Loftus, Róisín M.; Heinrich, Paul; Oefner, Peter J.; Lynch, Lydia; Gardiner, Clair M.; Dettmer, Katja & Finlay, David K.
  
• (2018) D-2-hydroxyglutarate interferes with HIF-1α stability skewing T-cell metabolism towards oxidative phosphorylation and impairing Th17 polarization. Oncoimmunology 7(7):e1445454
  Böttcher, Martin; Renner, Kathrin; Berger, Raffaela; Mentz, Kristin; Thomas, Simone; Cardenas-Conejo, Zugey Elizabeth; Dettmer, Katja; Oefner, Peter J.; Mackensen, Andreas; Kreutz, Marina & Mougiakakos, Dimitrios
  
• (2018) Double genetic disruption of lactate dehydrogenases A and B is required to ablate the "Warburg effect" restricting tumor growth to oxidative metabolism. The Journal of Biological Chemistry 293(41):15947–15961
  Ždralević, Maša; Brand, Almut; Di Ianni, Lorenza; Dettmer, Katja; Reinders, Jörg; Singer, Katrin; Peter, Katrin; Schnell, Annette; Bruss, Christina; Decking, Sonja-Maria; Koehl, Gudrun; Felipe-Abrio, Blanca; Durivault, Jérôme; Bayer, Pascale; Evangelista, Marie; O'Brien, Thomas; Oefner, Peter J.; Renner, Kathrin; Pouysségur, Jacques; ... & Kreutz, Marina
  
• (2018) Polyol pathway links glucose metabolism to the aggressiveness of cancer cells. Cancer Research 78(7):1604-1618
  Schwab, Annemarie; Siddiqui, Aarif; Vazakidou, Maria Eleni; Napoli, Francesca; Böttcher, Martin; Menchicchi, Bianca; Raza, Umar; Saatci, Özge; Krebs, Angela M.; Ferrazzi, Fulvia; Rapa, Ida; Dettmer-Wilde, Katja; Waldner, Maximilian J.; Ekici, Arif B.; Rasheed, Suhail Ahmed Kabeer; Mougiakakos, Dimitrios; Oefner, Peter J.; Sahin, Ozgur; Volante, Marco; ... & Ceppi, Paolo
  
• (2019) D-2-Hydroxyglutarate and L-2-hydroxyglutarate inhibit IL-12 secretion by human monocyte-derived dendritic cells. International Journal of Molecular Sciences 20(3):E742
  Ugele, Ines; Cárdenas-Conejo, Zugey Elizabeth; Hammon, Kathrin; Wehrstein, Monika; Bruss, Christina; Peter, Katrin; Singer, Katrin; Gottfried, Eva; Boesch, Jakob; Oefner, Peter; Dettmer, Katja; Renner, Kathrin & Kreutz, Marina