Analyses of the RNA polymerase I (Pol I) machinery: specific transcriptional mechanisms and comparison with other RNA polymerases (A01)

• Subject Area: Biochemistry
• Term: from 2011 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 185476941

RNA polymerase I (Pol I) is a specialized enzyme which transcribes the ribosomal RNA (rRNA) genes in all eukaryotes investigated so far. This project aims to unravel specific features of the Pol I machinery and to gain more insights into the mechanism of Pol I dependent transcription elongation and termination. Analyses of how structural and functional differences between the three eukaryotic nuclear RNA polymerases influence transcription of various nucleoprotein templates will be continued on a molecular level and will include Pol I mutants and templates of higher complexity.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 960: Ribosome Formation: Principles of RNP Biogenesis and Control of their Function
• Applicant Institution: Universität Regensburg
• Project Heads: Privatdozent Dr. Joachim Griesenbeck; Dr. Philipp Milkereit; Professor Dr. Herbert Tschochner