RNP complexes regulating the accessibility of higher order structures of chromatin (B02)

• Subject Area: Biochemistry
• Term: from 2011 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 185476941

Chromatin is stably associated with RNA and RNP complexes can open higher order structures of chromatin. We study the functional role of the RNA and the mechanisms of chromatin opening. We perform ChIP-Seq and RNA IPs of DF31 and HMGN5 to identify the chromatin targeting signals and test them in interaction assays. We will functionally address the chromatin opening mechanism with an in vivo GFP-HMGN5 targeting system and mutants of the protein. To study RNA dependent chromatin opening in a genome wide scale, we established the differential MNase treatment of cells combined with high-throughput sequencing and advanced bioinformatic analysis. We will continue to work on the development of the bioinformatical and statistical analysis of the large scale data sets and combine the assay with the functional testing of our model protein.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 960: Ribosome Formation: Principles of RNP Biogenesis and Control of their Function
• Applicant Institution: Universität Regensburg
• Project Head: Professor Dr. Gernot M. Längst