Mass spectrometry of proteins (Z01)

• Subject Area: Biochemistry
• Term: from 2011 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 185476941

In collaboration with different CRC projects we will apply quantitative proteomics methods such as SILAC, iTRAQ, Label-free, and further optimize workflows for our Bruker MaXis plus UHR-QTOF platform. Moreover, we will apply Selected Reaction Monitoring (SRM) as a very sensitive targeted proteomics approach for relative and absolute quantification of proteins and posttranslational modifications. We furthermore aim to implement emerging mass spectrometric methods such as Data-independent acquisition (DIA) for quantitative proteomics and Top-down proteomics for proteoform profiling.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 960: Ribosome Formation: Principles of RNP Biogenesis and Control of their Function
• Applicant Institution: Universität Regensburg
• Project Heads: Dr. Astrid Bruckmann, since 7/2019; Professor Dr. Rainer Deutzmann, until 6/2019