Project Details
Projekt
New Protein Chemical Tools for the Analysis of Cooperative Effects within Protein Interactions mediated by Ubiquitin-like Modifiers (B14)
Subject Area
Biochemistry
Term
from 2012 to 2021
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 104405829
Posttranslational modification of a substrate protein with the ubiquitin-like modifier SUMO equips the substrate protein with an additional protein interaction interface. Cooperative binding of a partner protein at each unit of the protein-SUMO conjugate results in the re-wiring of the cellular protein network. We will investigate both the orientation-dependent cooperativity and allosteric cooperativity of the protein binding interfaces and exploit these effects to identify new binding partners. Using new protein chemistry tools developed in this project as well as predictions from computational simulation provided in the research consortium, we will focus on the recognition of SUMO chains and the formation of the E3-ligase complexes relevant for SUMO transfer.
DFG Programme
Collaborative Research Centres
Applicant Institution
Universität Münster
Project Head
Professor Dr. Henning D. Mootz
