Multiplexing FRET-based Tension Sensors to Reveal Cell Adhesion Forces during Collective Cell Migration (B09)

• Subject Area: Biophysics ; Cell Biology
• Term: from 2012 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 111166240

In this project, we will apply a set of single-molecule calibrated tension sensor modules, which were developed and calibrated in the previous funding periods, to generate Förster resonance energy transfer (FRET)-based probes that report piconewton-scale forces across central components of integrin- and cadherin based adhesion structures. By combining these tension sensors with a recently established multiplexing approach, we will simultaneously investigate the molecular mechanics of cell-matrix and cell-cell adhesion during collective cell migration.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 863: Forces in Biomolecular Systems
• Applicant Institution: Technische Universität München (TUM)
• Project Head: Professor Dr. Carsten Grashoff