Analysis on the origin, function and regulation of B-chromosome located gene fragments
Final Report Abstract
The results of this project provided new insights into the transcriptional activity of the rye B chromosome and changed the prevalent view that B chromosomes (Bs) are silent genetic elements. We found that B chromosomes similar to A chromosomes (As) are interacting with active form of RNA polymerase II. We showed that genic sequences located on B chromosomes are transcriptional active. In addition, the transcriptional activity of the rye B was shown to be regulated in different tissues and with ability to modulate the transcriptional activity of A chromosomes in trans. Sequences comparison revealed that Bs have accumulated mutation in contrast to A-located genic sequences. To investigate the influence of B chromosomes on the transcriptome profile of rye and also to determine B-specific transcripts and those A-located genes which are differentially expressed in presence of Bs, RNAseq was performed using polyadenylated RNA from vegetative (leaf and root) and generative (anther) tissues of plants with and without Bs. We could show that presence of Bs increase transcriptional activity of repeat elements especially in generative tissues.
Publications
- A Selfish Supernumeray Chromosome Revealed its Origin as a Mosaic of Host Genome and Organellar Sequences, Plant and Animal Genome XX Conference, 14.01-18.01.2012, San Diego, USA
Martis, M., Klemme, S., Banaei-Moghaddam, A. M., Blattner, F. R., Macas, J., Schmutzer, T., Scholz, U., Gundlach, H., Wicker, T., Novak, P., Neumann, P., Simkova, H., Dolezel, J., Bauer, E., Haseneyer, G., Fuchs, J., Stein, N., Mayer, K. F. X., and Houben, A.
- Nondisjunction in favour of a chromosome- the mechanism of B chromosome accumulation. Gatersleben Research Conference, 23.04-25.04.2012, Gatersleben, Germany
Banaei Moghaddam, A.M., Klemme, S, Kumke, K, Schubert, V, Nagaki, K, and Houben, A.
- (2013). Formation and expression of pseudogenes on the B chromosome of rye. Plant Cell 25, 2536-2544
Banaei-Moghaddam, A.M., Meier, K., Karimi-Ashtiyani, R., and Houben, A.
(See online at https://doi.org/10.1105/tpc.113.111856) - Nondisjunction in favor of a chromosome: Possible implication of noncoding RNAs. Non-coding RNA in plants conference, 10.07-12.07.2013, Leucorea Wittenberg, Germany
Banaei-Moghaddam A. M., Carchilan M., Marques A., Klemme S. and Houben A.
- (2014). Evolution and biology of supernumerary B chromosomes. Cellular and Molecular Life Sciences 71, 467-478
Houben, A., Banaei-Moghaddam, A.M., Klemme, S., and Timmis, J.N.
(See online at https://doi.org/10.1007/s00018-013-1437-7) - Genes on B chromosomes: old questions revisited with new tools. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
Volume 1849, Issue 1, January 2015, Pages 64-70
Banaei-Moghaddam, A.M., Martis, M.M., Macas, J., Gundlach, H., Himmelbach, A., Altschmied, A., Mayer, K.F.X., and Houben, A.
(See online at https://doi.org/10.1016/j.bbagrm.2014.11.007)
