Imaging and modelling of seed metabolism in oilseed rape
Final Report Abstract
ln this project we tested our hypothesis that there are metabolically distinct regions in the embryo, which are governed by distinct metabolic networks and pathway activities, respectively. We uncovered this metabolic compartmentation by application of non-invasive nuclear magnelic resonance (NMR), metabolite analysis and modelling approaches. Three-dirmensional models of seed at different developmental stages were generated demonstrating the relation between storage processes, oil deposition and seed structure. We were able to generate an appropriate experimental dataset to apply flux balance analysis; its application to the developrng embryos explains the local differences ibn metabolic activity. The outcome of the project also involves the development of integrative tools for the non-invasive analysis of plant seeds, capable to assist and facilitate research on crop improvement. We are expecting great impact of our work on the general understanding of seed biology and on spreading new ideas for application of NMR and noninvasive modelling approaches in plant biology.
Publications
- (2013). Seed architecture shapes embryo metabolism in oilseed rape. Plant Cell 25: 1625-1640
Borisjuk L, Neuberger T, Schwender J, Heinzel N, Sunderhaus S, Fuchs J, Hay JO, Tschiersch H, Braun HP, Denolf P, Lambert B, Jakob PM, Rolletschek H
(See online at https://doi.org/10.1105/tpc.113.111740) - (2013). Void space inside the developing seed of Brassica napus and the modelling of its function. New Phytologist 199: 936-947
Verboven P, Herremans E, Borisjuk L, Helfen L, Ho QT, Tschiersch H, Fuchs J, Nicolaï BM, Rolletschek H
(See online at https://doi.org/10.1111/nph.12342) - (2014). B. napus seed endosperm – metabolism and signaling in a dead end tissue. Journal of Proteomics 108: 382-426
Lorenz C, Rolletschek H, Sunderhaus S, Braun HP
(See online at https://doi.org/10.1016/j.jprot.2014.05.024) - (2014). Integration of a constraint-based metabolic model of Brassica napus developing seeds with 13C-Metabolic Flux Analysis. Frontiers in Plant Science 5: 724
Hay, J.O., Shi, H., Heinzel, N., Hebbelmann, I., Rolletschek, H., Schwender, J.
(See online at https://doi.org/10.3389/fpls.2014.00724) - (2014). Transcript abundance on its own cannot be used to infer fluxes in central metabolism. Frontiers in Plant Science 5:668
Schwender J, König C, Klapperstück M, Heinzel N, Munz E, Hebbelmann I, Denolf P, De Bodt S, Redestig H, Caestecker E, Jakob PM, Borisjuk L, Rolletschek H
(See online at https://doi.org/10.3389/fpls.2014.00668) - (2015). Quantitative multilevel analysis of central metabolism in developing oilseeds of Brassica napus during in vitro culture. Plant Physiology 168: 828-848
Schwender J, Hebbelmann I, Heinzel N, Hildebrandt T, Naik D, Rogers A, Klapperstück M, Braun H- P, Schreiber F, Denolf, P, Borisjuk L, Rolletschek H
(See online at https://doi.org/10.1104/pp.15.00385)
