RNA elements and protein factors required for pre-ribosome remodelling during 60S biogenesis in yeast (A12)

• Subject Area: Biochemistry
• Term: from 2012 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 105286809

Ribosome biogenesis in eukaryotes is a major cellular pathway that requires multiple cofactors, among them DEAD/H-box RNA helicases. We have identified binding sites of multiple large subunit RNA helicases on yeast ribosomal RNAs and analyse their molecular functions in the pathway. Our data also reveal interactions between several helicases and non-ribosomal RNAs, suggesting that these proteins perform additional functions in other pathways. Understanding the molecular functions and regulation of RNA helicases as well as their interplay will shed light on key steps in RNA metabolism and the cross-regulation of different cellular processes.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 860: Integrative Structural Biology of Dynamic Macromolecular Assemblies
• Applicant Institution: Georg-August-Universität Göttingen
• Project Head: Professor Dr. Markus T. Bohnsack