The miRNA and single cell perspectives of the E2F1/inflammation/autophagy network in human adipose tissue (B02)

• Subject Area: Biochemistry ; Endocrinology, Diabetology, Metabolism
• Term: since 2013
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 209933838

Autophagy plays an important role in the development of adipose tissue dysfunction. In human obesity, activation of autophagy is regulated by E2F1 and specific miRNAs. The project now takes the E2F1-autophagy/inflammation network to the single cell level. Single-nucleus RNA-sequencing (sNucSeq) will be used to capture the cellular repertoire, and the single cell-type impact of the E2F1 network. The project further aims to understand how E2F1 signifies changes in the repertoire of adipose tissue miRNAs, and to what degree these are reflected in circulating miRNAs. Finally, it will be determined if these E2F1 network components constitute biomarkers to predict clinical outcomes in the response to bariatric surgery, such as weight loss, metabolic improvement, diabetes remission, and decrease in cardiovascular risk parameters.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1052: Obesity Mechanisms
• International Connection: Israel
• Applicant Institution: Universität Leipzig
• Project Head: Professor Dr. Assaf Rudich