Role of the recognition molecule L1 in stem cell differentiation and stem cell based therapy in the mouse

• Applicant: Professorin Dr. Melitta Schachner
• Subject Area: Developmental Neurobiology
• Term: from 2006 to 2012
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 23324403

• Final Report Year: 2012

Final Report Abstract

Wir haben in mehreren Ansätzen zeigen koennen, dass das neurale Zellerkennungmolekül L1 die Regeneration fördert in verschiedenen Mausmodellsystemen für neurologische Krankheiten beim Menschen. Dies konnte für Mäuse Rückenmarksgeschädigte und somit gelähmte Tiere als auch für Tiere mit Phänotypen der Parkinsonschen und Huntingtonschen Krankheit gezeigt werden anhand von neural eindifferenzierten embryonalen Stammzellen der Maus. Das L1 Molekül ist somit ein Kandidat für therapeutische Ansätze beim Menschen.

Publications

• (2007). Adeno-associated virus-mediated L1 expression promotes functional recovery after spinal cord injury. Brain 130, 954-969
  Chen, J., Wu, J., Apostolova, I., Skup, M., Irintchev, A., Kugler, S., and Schachner, M.
  (See online at https://doi.org/10.1093/brain/awm049)
• (2010). Embryonic stem cell-derived L1 overexpressing neural aggregates enhance recovery in Parkinsonian mice. Brain 133, 189-204
  Cui, Y.F., Hargus, G., Xu, J.C., Schmid, J.S., Shen, Y.Q., Glatzel, M., Schachner, M., and Bernreuther, C.
  (See online at https://doi.org/10.1093/brain/awp290)
• (2010). Schwann cells engineered to express the cell adhesion molecule L1 accelerate myelination and motor recovery after spinal cord injury. Exp. Neurol. 221, 201-216
  Lavdas, A.A., Chen, J., Papastefanaki, F., Chen, S., Schachner, M., Matsas, R., and Thomaidou, D.
  (See online at https://doi.org/10.1016/j.expneurol.2009.10.024)
• (2011). Adhesion molecule L1 overexpressed under the control of the neuronal Thy-1 promoter improves myelination after peripheral nerve injury in adult mice. Exp. Neurol. 229, 339-352
  Guseva, D., Zerwas, M., Xiao, M., Jakovcevski, I., Irintchev, A., and Schachner, M.
  (See online at https://doi.org/10.1016/j.expneurol.2011.02.018)
• (2011). Embryonic stem cell-derived L1 overexpressing neural aggregates enhance recovery after spinal cord injury in mice. PLoS One 6, e17126
  Cui, Y.F., Xu, J.C., Hargus, G., Jakovcevski, I., Schachner, M., and Bernreuther, C.
  (See online at https://doi.org/10.1371/journal.pone.0017126)
• (2012) In vitro generation of three-dimensional substrate-adherent embryonic stem cell-derived neural aggregates for application in animal models of neurological disorders. Curr. Protoc. Stem Cell Biol. 2012 May; Chapter 2:Unit 2D.11
  Hargus, G., Cui, Y.F., Dihné, M., Bernreuther, C., and Schachner, M.
  (See online at https://doi.org/10.1002/9780470151808.sc02d11s21)
• (2012) Promotion of spinal cord regeneration by neural stem cell-secreted trimerized adhesion molecule L1. PloS One 7:e46223
  He, X., Knepper, M., Ding, C., Li, J., Castro, S., Siddiqui, M., and Schachner, M.
  (See online at https://doi.org/10.1371/journal.pone.0046223)