Project Details
Projekt Print View

Protonation and conformational changes at the surface of proteins (A02)

Subject Area Biophysics
Term since 2013
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 221545957
 
Advanced fluorescence spectroscopic methods in combination with molecular biology are applied to understand the interplay between activation of the embedded cofactor and protonation, conformational and hydration dynamics at the protein surface of cytochrome c oxidase (CcO) and the photoreceptor phytochrome. We will address the enigmatic proton release pathways in CcO using probes attached to surface residues and internal groups such as R473. Vibrational Stark Effect probes at single cysteine mutants for electric field changes and proton release and uptake studies at the protein surface will facilitate the understanding of signal transfer in phytochromes.
DFG Programme Collaborative Research Centres
Applicant Institution Freie Universität Berlin
Project Heads Professorin Dr. Ulrike Alexiev, until 12/2023 (†); Professor Dr. Joachim Heberle, since 12/2023
 
 

Additional Information

Textvergrößerung und Kontrastanpassung