Advanced adaptive optics instrumentation for ophthalmic imaging and function testing: the single cell approach to visual function and dysfunction
Final Report Abstract
A new Emmy Noether-Research group for advanced adaptive optics instrumentation for fundamental neuroscience and clinical applications, the AO-Vision-Laboratory, has been established at the Department of Ophthalmology, University of Bonn. Although a delay in system development was introduced due to legal regulations for medical devices, we could meet the originally planned research aims with cost neutral term extensions. In proof-of-principle experiments, we demonstrate the capabilities of cone-targeted, single-cell retinal function testing, with important implications for high-resolution imagery and their interpretation in clinical scenarios. We show that the human trichromatic mosaic can be successfully mapped psychophysically, and uncover the role of eye micro movements in spatial vision tasks. Detailed anatomical and functional characterizations of the human fovea, the centre of the human retina, were carried out. A new clinical software/hardware interface (IGUIDE) has been developed that will boost AOSLO imaging in the clinic. Important research partnerships have been established locally, nationally and internationally, that promise exciting new avenues for the continuation of micro-psychophysics research within the research landscape in Germany and abroad.
Publications
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(2014) Mapping the perceptual grain of the human retina. The Journal of Neuroscience, 34(16):5667-5677
Harmening WM, Tuten WS, Roorda A, Sincich LC
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(2015) Normal perceptual sensitivity arising from weakly reflective cone photoreceptors. Investigative Ophthalmology & Visual Science, 56(8):4431-4438
Bruce KS, Harmening WM, Langston BR, Tuten WS, Roorda A, Sincich LC
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(2016) Functional Imaging of Cone Photoreceptors. In: Baraas R, Marshall J, Kremers J (eds.) Human Color Vision. Volume 5 of the Springer Series in Vision Research. pp 71-104
Sincich LC, Sabesan R, Tuten WS, Roorda A, Harmening WM
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(2017) Benefits of retinal image motion at the limits of spatial vision. Journal of Vision, 17(1): 1-11
Ratnam K, Domdei N, Harmening WM, Roorda A
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(2017) Spatiochromatic interactions between individual cone photoreceptors in the human retina. The Journal of Neuroscience, 37(39): 9498-9509
Tuten WS, Harmening WM, Sabesan R, Roorda A, Sincich LC
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(2018) Ultra-high contrast retinal display system for single photoreceptor psychophysics. Biomedical Optics Express, 9: 157-172
Domdei N, Domdei L, Reiniger J, Linden M, Holz FG, Roorda A, Harmening WM
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(2019) Adaptive Optics for Photoreceptor-Targeted Psychophysics. High resolution Imaging in Microscopy and Ophthalmology, Springer, Cham. pp 359-375
Harmening WM, Sincich LC
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(2019) Eye tracking-based estimation and compensation of chromatic offsets for multi-wavelength retinal microstimulation with foveal cone precision. Biomedical Optics Express, 10:4126-4141
Domdei N, Linden M, Reiniger JL, Holz FG, Harmening WM
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(2019) Habitual higher order aberrations affect Landolt but not Vernier acuity. Journal of Vision, 19(5): 1-15
Reiniger JL, Lobecke AC, Sabesan R, Bach M, Verbakel F, de Brabander J, Holz FG, Berendschot TTJM, Harmening WM
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(2020) Effect of cone spectral topography on chromatic detection sensitivity. Journal of the Optical Society of America A, 37(4): A244-A254
Neitz A, Jiang X, Kuchenbecker JA, Domdei N, Harmening WM, Yan H, Yeonan-Kim, Patterson SS., Neitz M, Neitz J, Coates DR, Sabesan R
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(2021) Foveal vision. Current Biology, 31(11):R701-R7
Tuten WS, Harmening WM
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(2021) Human gaze is systematically offset from the center of cone topography. Current Biology, 31(18): P4188-4193
Reiniger JL, Domdei N, Holz FG, Harmening WM
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(2021) The relationship between visual sensitivity and eccentricity, cone density an outer segment length in the human foveola. Investigative Ophthalmology & Visual Science, 62 (9):1-16
Domdei N, Reiniger JL, Holz FG, Harmening WM
