Domino nucleosome remodeling complexes and histone H2A.V dynamics in transcription and DNA repair (A01)

• Subject Area: General Genetics and Functional Genome Biology
• Term: since 2013
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 213249687

The Drosophila DOM proteins are SWR1-type ATPases involved in exchanging the histone variant H2A.V. The two DOM splice isoforms that reside in different complexes with distinct functions. The DOM-B complex catalyses the bulk of H2A.V incorporation akin to the yeast SWR1 complex. The DOM-A complex contains a TIP60 acetyltransferase module, resembling yeast NuA4. Both complexes regulate transcription and distinctly contribute to the DNA damage response (DDR). We study the potential crosstalk between the transcriptional and DDR-related activities of both complexes, their targeting to promoters and DNA breaks and their enzymatic activities (histone exchange und acetylation), intrinsic and at target sites, under normal and DNA damage conditions.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1064: Chromatin Dynamics
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Professor Dr. Peter Burkhard Becker