Strukturelle Untersuchungen des Aquarius Proteins und assoziierter Spleißfaktoren, welche das prä-mRNA Spleißen mit nachfolgenden Schritten der RNA-Prozessierung koppeln

• Antragsteller: Vladimir Pena, Ph.D.
• Fachliche Zuordnung: Strukturbiologie
• Förderung: Förderung von 2013 bis 2021
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 241796087

• Erstellungsjahr: 2022

Zusammenfassung der Projektergebnisse

It has long been assumed that splicing depends on eight RNA helicases. Here we show that Aquarius is also essential for splicing. Furthermore, we show that catalytic activation occurs in two steps, one driven by the RNA helicase PRP2, and the second one driven by Aquarius. We resolve the structure of the BAQR spliceosome, found between the action of the two helicases, thereby providing deep insight into the catalytic activation in pre-mRNA splicing.

Projektbezogene Publikationen (Auswahl)

• (2015). Structural investigation of the spliceosomal helicase Aquarius, Univ. of Goettingen
  Inessa De
  
• (2015). The RNA helicase Aquarius exhibits structural adaptations mediating its recruitment to spliceosomes. Nat. Struct. Mol. Biol. 22(2):138-44
  De, Inessa; Bessonov, Sergey; Hofele, Romina; dos Santos, Karine; Will, Cindy L; Urlaub, Henning; Lührmann, Reinhard & Pena, Vladimir
  
• (2016). The organization and contribution of helicases to RNA splicing. WIREs RNA. 7(2): 259-274
  De, Inessa; Schmitzová, Jana & Pena, Vladimir
  
• (2022). An integrated model for termination of RNA polymerase III transcription. Science Advances
  Xie, Juanjuan; Aiello, Umberto; Clement, Yves; Haidara, Nouhou; Girbig, Mathias; Schmitzova, Jana; Pena, Vladimir; Müller, Christoph W.; Libri, Domenico & Porrua, Odil