Acetylation of SUMO paralogs as a determinant of SUMO-mediated protein networks

• Applicant: Professor Dr. Stefan Müller
• Subject Area: Cell Biology ; Biochemistry
• Term: from 2013 to 2017
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 242915414

• Final Report Year: 2021

Final Report Abstract

In summary, our project revealed novel insight into SUMO signaling and the interconnection of SUMO with acetylation and the SIRT1 pathway. Even though we had to adapt some of our initial plans, we feel that the project was very productive as demonstrated by a series of highly visible publications. The work on SUMO chains, which was initiated in the course of this project, has now also led to important findings with in the fields of neurodegeneration and tumor biology. The basic findings on SUMO chain signaling in the DDR lead to a very fruitful collaboration with Prof. Keller (Charité Berlin), which will ultimately open the path for novel treatment in B cell lymphoma.

Publications

• (2014) The SUMO-specific isopeptidase SENP3 regulates MLL1/MLL2 methyltransferase complexes and controls osteogenic differentiation. Mol Cell 55: 47-58
  Nayak A, Viale-Bouroncle S, Morsczeck C, Müller S
  (See online at https://doi.org/10.1016/j.molcel.2014.05.011)
• (2014). mTOR signaling regulates nucleolar targeting of the SUMO-specific isopeptidase SENP3. Mol Cell Biol 34: 4474-4484
  Raman N, Nayak A, and Müller S
  (See online at https://doi.org/10.1128/MCB.00801-14)
• (2016) The AAA ATPase MDN1 acts as a SUMO-targeted regulator in mammalian pre-ribosome remodeling. Mol Cell 64: 607-615
  Raman N, Weir E, Müller S
  (See online at https://doi.org/10.1016/j.molcel.2016.09.039)
• (2017) Site-specific inhibition of the small ubiquitin-like modifier (SUMO)-conjugating enzyme Ubc9 selectively impairs SUMO chain formation. J Biol Chem 292: 15340-15351
  Wiechmann S, Gartner A, Kniss A, Stengl A, Behrends C, Rogov VV, Rodriguez MS, Dotsch V, Müller S, Ernst A
  (See online at https://doi.org/10.1074/jbc.M117.794255)
• (2018) Acetylation of SUMO2 at lysine 11 favors the formation of non-canonical SUMO chains. EMBO Rep 19: pii: e46117
  Gärtner A, Wagner K, Hölper S, Kunz K, Rodriguez MS, Müller S
  (See online at https://doi.org/10.15252/embr.201846117)
• (2019) The SUMO isopeptidase SENP6 functions as a rheostat of chromatin residency in genome maintenance and chromosome dynamics. Cell Rep., 29:480-494.e5
  Wagner K, Kunz K, Piller T, Tascher G, Hölper S, Stehmeier P, Keiten-Schmitz J, Schick M, Keller U, Müller S
  (See online at https://doi.org/10.1016/j.celrep.2019.08.106)