The role of the N-end rule pathway in controlling plant response to the environment
Final Report Abstract
Manipulation of plants to provide stability of yield under unpredictable growth conditions will be essential to respond to the effect of climate change in increasing the uncertainty of the agricultural environment. The aim of the N-vironment project was to provide a mechanistic understanding of the role of the N-end rule pathway of targeted proteolysis in controlling plant responses to the environment. The project brought together six research groups with complementary expertise in fundamental molecular plant science, biochemistry and chemistry, in four institutions. The objectives of N-vironment were related to understand the role of this pathway in controlling plant-environment interactions, including defining the substrates and enzymatic components of the N-end rule pathway, understanding integration into cellular signaling pathways, and defining the extent of the role of the N- end rule pathway in plant response to the biotic and abiotic environment. During the project many proteins have been assessed as possible substrates, leading to the identification of a cohort of confirmed substrates of the Cys-Arg/N-end rule pathway, and association of the pathway to previously unknown developmental, physiological and environmental interactions were made. Analyses of phenotypes of mutant substrates and enzymic components of the pathway have shown the very wide range of abiotic and biotic stresses that are sensed by the pathway. Resources that we have developed during the project will allow future detailed dissection of the role of this pathway in controlling plant biology.
Publications
- (2014) Large-scale identification of gibberellinrelated transcription factors defines Group VII ERFs as functional DELLA partners. Plant Physiology, 166: 1022-1032
Marin-de la Rosa, N., Sotillo, B., Miskolczi, P., Gibbs, D.J., Vicente, J., Carbonero, P., Onate-Sanchez, L., Holdsworth, M.J., Bhalerao, R., Alabadi, D., Blazquez, M.A.
(See online at https://doi.org/10.1104/pp.114.244723) - (2014) Plant cysteine oxidases control the oxygen-dependent branch of the N-end-rule pathway. Nat Comm. 3425
Weits Da, Giuntoli B, Kosmacz M, Parlanti S, Hubberten H-M, Riegler H, Hoefgen R, Perata P, van Dongen JT, Licausi F
(See online at https://doi.org/10.1038/ncomms4425) - (2015) Constitutively expressed ERF-VII transcription factors redundantly activate the core anaerobic response in Arabidopsis thaliana. Plant Sciences. 236:37-43
Bui LT, Giuntoli B, Kosmacz M, Parlanti S, Licausi F
(See online at https://doi.org/10.1016/j.plantsci.2015.03.008) - (2015) Group VII Ethylene Response Factors co-ordinate oxygen and nitric oxide signal transduction and stress responses in plants. Plant Physiology 169: 23–31
Gibbs, D.J., Vicente Conde, J., Berckhan, S., Mendiondo, G.M., Prasad, G., Holdsworth M.J.
(See online at https://doi.org/10.1104/pp.15.00338) - (2015) How cells coordinate waste removal through their major proteolytic pathways. Nat. Cell Biol. 30, 841-842
Martens, S., Bachmair, A.
(See online at https://doi.org/10.1038/ncb3198) - (2015) Oxygen sensing and signaling. Annu Rev Plant Biol. 2015;66:345-67
van Dongen JT, Licausi F
(See online at https://doi.org/10.1146/annurev-arplant-043014-114813) - (2015) Seedling germination: seedlings follow sunshine and fresh air. Curr. Biol. 25, R565-R566
Potuschak, T., Bachmair, A.
(See online at https://doi.org/10.1016/j.cub.2015.05.001) - (2015) The stability and nuclear localization of the transcription factor RAP2. 12 are dynamically regulated by oxygen concentration. Plant, Cel & Environment. 38: 1094-1103
Kosmacz M, Parlanti S, Schwarzländer M, Kragler F, Licausi F, van Dongen JT
(See online at https://doi.org/10.1111/pce.12493) - (2016) Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot. BMC Plant Biology 16(1):251
Gravot, A., Richard, G., Lime, T., Lemarié, S., Jubault, M., Lariagon C., Lemoine J., Vincente, J., Robert- Seilaniantz, A., Holdsworth, M.J., Manzanares-Dauleux M.J.
(See online at https://doi.org/10.1186/s12870-016-0941-y) - (2017) Plant, Cell & Environment. Age‐dependent regulation Age‐dependent regulation of ERF‐VII transcription factor activity in Arabidopsis thaliana. Plant, Cell & environment
Giuntoli B, Shukla V, Maggiorelli F, Giorgi FM, Lombardi L, P Perata, Licausi F
(See online at https://doi.org/10.1111/pce.13037) - (2017) The Cys-Arg/N-end rule pathway is a general sensor of abiotic stress in flowering plants. Current Biology
Vicente, J., Mendiondo, G.G., Movahedi, M., Peirats- Llobet, M., Juan, Y., Shen, Y., Dambire, C., Smart, K., Rodriguez, P.L., Charng, Y., Gray, J.E., Holdsworth, M.J.
(See online at https://doi.org/10.1016/j.cub.2017.09.006)
