Planctomycetale Zellteilung: Molekulare Grundlagen der Knospung von Planctomyces limnophilus
Stoffwechselphysiologie, Biochemie und Genetik der Mikroorganismen
Zusammenfassung der Projektergebnisse
Taken together the project turned out exceptionally successful. Although, major objectives had to be modified due to funding, and due to the early unexpected discovery of peptidoglycan, the project resulted in two publications in Nature Communications. The main finding of this project is the peptidoglycan cell wall of Planctomycetes. Furthermore, Planctomycetes were found to be similar, yet distinct, compared to canonical Gram-negative bacteria. They comprise an enlarged periplasmic space but mostly lack the postulated compartmentalization of the cytosol. Nor do they perform an endocytosis-like uptake of proteins. In this project time-lapse microscopy and microfluidic methods were optimized for Planctomycetes. First experiment point towards a role of MreB in the cell division of some Planctomycetes. More research is needed to understand the division of these unusual bacteria.
Projektbezogene Publikationen (Auswahl)
- 2015. Planctomycetes do possess peptidoglycan. Nature Communications. 6: p.7116
Jeske O, Schüler M, Schumann P, Schneider A, Boedeker C, Jogler M, Rohde M, Mayer C, Engelhardt H, Spring S, Jogler C
(Siehe online unter https://doi.org/10.1038/ncomms8116) - 2016. Fuerstia marisgermanicae gen. nov., sp. nov., an unusual member of the phylum Planctomycetes from the German Wadden Sea. Frontiers in Microbiology. 22;7: 2079
Kohn T, Jogler M, Heuer A, Vollmers J, Boedeker C, Bunk B, Rast P, Borchert D, Glöckner I, Freese H, Overmann J, Rohde M, Kaster A, Wiegand S and Jogler C
(Siehe online unter https://doi.org/10.3389/fmicb.2016.02079) - 2017. Determining the bacterial cell biology of Planctomycetes. Nature Communications. 10;8: 14853
Boedeker C, Schüler M, Reintjes G, Jeske O, van Teeseling MCF, Jogler M, Rast P, Borchert D, Devos DP, Kucklick M, Schaffer M, Kolter R, van Niftrik L, Engelmann S, Amann R, Rohde M, Engelhardt H and Jogler C
(Siehe online unter https://doi.org/10.1038/ncomms14853)
