Nucleic-Acid Based Super-Resolution Microscopy

• Applicant: Professor Dr. Ralf Jungmann
• Subject Area: Biophysics
• Term: from 2014 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 251122678

• Final Report Year: 2021

Final Report Abstract

In conclusion, the research carried out through the Emmy Noether Grant has help tremendously to push DNA-based super-resolution microscopy to the next level, paving the way to quantitative high-throughput studies under biomedically relevant conditions. While we were able to push spatial resolution to below 5 nm as well as improve the labeling probes and image acquisition speed, there is still a dire need in carefully benchmarked, improved labeling probes for next-generation super-resolution microscopy, an area where we actively develop new approaches at the moment. Furthermore, we are pursuing applications of improved DNA-PAINT microscopy to cell-biological and biomedical questions such as the multiplexed visualization of cell surface receptor patterns with single protein resolution.

Publications

• Fast, background-free DNA-PAINT imaging using FRET-based probes Nano Letters (2017). 17(10): 6428-6434
  Auer, Alexander; Strauss, Maximilian T.; Schlichthaerle, Thomas & Jungmann, Ralf
  
• Multiplexed 3D super-resolution imaging of whole cells using Spinning Disk Confocal Microscopy and DNA- PAINT Nature Communications (2017). 8: 2090
  Schueder, Florian; Lara-Gutiérrez, Juanita; Beliveau, Brian J.; Saka, Sinem K.; Sasaki, Hiroshi M.; Woehrstein, Johannes B.; Strauss, Maximilian T.; Grabmayr, Heinrich; Yin, Peng & Jungmann, Ralf
  
• Super-Resolution Microscopy with DNA-PAINT Nature Protocols (2017). 12: 1198-1228
  Schnitzbauer, Joerg; Strauss, Maximilian T; Schlichthaerle, Thomas; Schueder, Florian & Jungmann, Ralf
  
• Universal Super-Resolution Multiplexing by DNA Exchange Angewandte Chemie Int. Ed. (2017). 56: 4052-4055
  Schueder, Florian; Strauss, Maximilian T.; Hoerl, David; Schnitzbauer, Joerg; Schlichthaerle, Thomas; Strauss, Sebastian; Yin, Peng; Harz, Hartmann; Leonhardt, Heinrich & Jungmann, Ralf
  
• Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging Nature Methods (2018). 15: 685–688
  Strauss, Sebastian; Nickels, Philipp C.; Strauss, Maximilian T.; Jimenez Sabinina, Vilma; Ellenberg, Jan; Carter, Jeffrey D.; Gupta, Shashi; Janjic, Nebojsa & Jungmann, Ralf
  
• Quantifying absolute addressability in DNA origami with molecular resolution Nature Communications (2018). 9: 1600
  Strauss, Maximilian T.; Schueder, Florian; Haas, Daniel; Nickels, Philipp C. & Jungmann, Ralf
  
• Site-specific labeling of Affimers for DNA-PAINT microscopy Angewandte Chemie Int. Ed. (2018). 57: 11060-11063
  Schlichthaerle, Thomas; Eklund, Alexandra S.; Schueder, Florian; Strauss, Maximilian T.; Tiede, Christian; Curd, Alistair; Ries, Jonas; Peckham, Michelle; Tomlinson, Darren C. & Jungmann, Ralf
  
• 124-color super-resolution imaging by engineering DNA-PAINT blinking kinetics Nano Letters (2019). 19(4): 2641-2646
  Wade, Orsolya K.; Woehrstein, Johannes B.; Nickels, Philipp C.; Strauss, Sebastian; Stehr, Florian; Stein, Johannes; Schueder, Florian; Strauss, Maximilian T.; Ganji, Mahipal; Schnitzbauer, Joerg; Grabmayr, Heinrich; Yin, Peng; Schwille, Petra & Jungmann, Ralf
  
• An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions Nature Methods (2019). 16: 1101-1104
  Schueder, Florian; Stein, Johannes; Stehr, Florian; Auer, Alexander; Sperl, Bianca; Strauss, Maximilian T.; Schwille, Petra & Jungmann, Ralf
  
• Direct visualization of single nuclear pore complex proteins using genetically-encoded probes for DNA- PAINT Angewandte Chemie Int. Ed. (2019). 58: 13004-13008
  Schlichthaerle, Thomas; Strauss, Maximilian T.; Schueder, Florian; Auer, Alexander; Nijmeijer, Bianca; Kueblbeck, Moritz; Jimenez Sabinina, Vilma; Thevathasan, Jervis V.; Ries, Jonas; Ellenberg, Jan & Jungmann, Ralf
  
• Peptide-PAINT super-resolution imaging using transient coiled coil interactions Nano Letters (2020). 20(09): 6732-6737
  Eklund, Alexandra S.; Ganji, Mahipal; Gavins, Georgina; Seitz, Oliver & Jungmann, Ralf
  
• Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT Nature Methods (2020). 17: 78-791
  Strauss, Sebastian & Jungmann, Ralf