Project Details
Projekt
Pathogen passage through the Nuclear Pore Complex (07)
Subject Area
Cell Biology
Term
from 2014 to 2022
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 240245660
We aim to decipher the molecular structure and dynamics of the actual pathogen mediated transport mechanism through the nuclear pore complex (NPC) by focusing on two different viral systems, HIV and HBV. We will employ state of the art single molecule and super-resolution microscopy techniques to visualize protein flexibility and pro-tein-protein interactions between the pathogen and the NPC with high spatial (molecular/nanometer) and temporal resolution. Fundamental to such high resolution approaches is to minimally invasively engineer proteins site specifi-cally with ultra-photo stable, small fluorescent dyes. Technologies utilizing combined genetic code expansion and click technology to achieve this will be further developed in this project.
DFG Programme
Collaborative Research Centres
Applicant Institution
Ruprecht-Karls-Universität Heidelberg
Project Head
Professor Dr. Edward A. Lemke
