Molecular bases for the role of IFT172 in ciliogenesis and in ciliopathy

• Applicants: Dr. Esben Lorentzen; Dr. Naoko Mizuno
• Subject Area: Biochemistry ; Structural Biology ; Cell Biology
• Term: from 2014 to 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 258711904

• Final Report Year: 2018

Final Report Abstract

Given its very large size of 200kDa, IFT172 has been very difficult to study due to problems in protein expression and purification. With support from the DFG, we have now managed to carry out detailed characterizations of IFT172 that highlight its ancestral function in lipid binding and discover new function in membrane remodeling.

Publications

• (2018) Membrane association and remodeling by intraflagellar transport protein IFT172. Nature communications 9 (1) 4684
  Wang, Qianmin; Taschner, Michael; Ganzinger, Kristina A.; Kelley, Charlotte; Villasenor, Alethia; Heymann, Michael; Schwille, Petra; Lorentzen, Esben; Mizuno, Naoko
  (See online at https://doi.org/10.1038/s41467-018-07037-9)
• (2016) Intraflagellar transport proteins 172, 80, 57, 54, 38, and 20 form a stable tubulin-binding IFT-B2 complex. EMBO J 35(7):773-90
  Taschner M., Weber K., Mourão A., Vetter M, Awasthi M., Stiegler M., Bhogaraju S, Lorentzen E.
  (See online at https://doi.org/10.15252/embj.201593164)