Interaction of sulfur and nitrogen metabolism: significance of different serine sources for cysteine biosynthesis
Final Report Abstract
Taken together, our data support the hypothesis that in leaves of the C3 plant species Arabidopsis thaliana the photorespiratory pathway indeed represents the predominant source of Ser for Cys biosynthesis, with some possible minor contribution of plastidial SHMT when the activity of glycine decarboxylase is impaired, as in the bou-2 mutant. The phosphorylated pathway plays a rather minor role in leaves. Even under enhanced demand for reduced sulfur, such as Cd treatment, the contribution of the phosphorylated pathway was rather minor. We could however not exclude the root as a source of Ser via long-distance transport by the phloem in lines with impaired GDC activity. Both, altered GDC and PSP activity were associated with rather complex changes of the transcriptome, indicating altered regulation of C and N metabolism, which also affects sulfur metabolism. Further dissection of these interactions would require inducible repression of BOU and/or PSP, followed by a time-series analysis of transcriptomic and metabolomic changes.
Publications
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(2015). Overexpression of γ-glutamylcysteine synthetase mediates changes in cadmium influx, allocation, and detoxification in poplar. New Phytol 205: 240-254
He, Jiali; Li, Hong; Ma, Chaofeng; Zhang, Yanli; Polle, Andrea; Rennenberg, Heinz; Cheng, Xingqi & Luo, Zhi‐Bin
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(2015). Nitrogen-fixing nodules as a new strong source of reduced sulfur trigger global changes in sulfur metabolism in Lotus japonicus. Plant Cell 27: 2384-2400
Kalloniati, Chrysanthi; Krompas, Panagiotis; Karalias, Georgios; Udvardi, Michael K.; Rennenberg, Heinz; Herschbach, Cornelia & Flemetakis, Emmanouil
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(2016) Manipulating photorespiration to increase plant productivity: recent advances and perspectives for crop improvement. J Exp Bot, 67(10): 2977-88
Betti, Marco; Bauwe, Hermann; Busch, Florian A.; Fernie, Alisdair R.; Keech, Olivier; Levey, Myles; Ort, Donald R.; Parry, Martin A. J.; Sage, Rowan; Timm, Stefan; Walker, Berkley & Weber, Andreas P. M.
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(2016) Perspectives for a better understanding of the metabolic integration of photorespiration within a complex plant primary metabolism network. J Exp Bot, 67(10): 3015-26
Hodges, Michael; Dellero, Younès; Keech, Olivier; Betti, Marco; Raghavendra, Agepati S.; Sage, Rowan; Zhu, Xin-Guang; Allen, Doug K. & Weber, Andreas P.M.
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(2016) Photorespiration: origins and metabolic integration in interacting compartments. J Exp Bot, 67 (10), 2915-2918
Hagemann, Martin; Weber, Andreas PM & Eisenhut, Marion
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(2016) Photorespiration is crucial to the dynamic response of photosynthetic metabolism and stomatal movement to altered CO2 availability. Mol Plant 10(1):47-61
Eisenhut, Marion; Bräutigam, Andrea; Timm, Stefan; Florian, Alexandra; Tohge, Takayuki; Fernie, Alisdair R.; Bauwe, Hermann & Weber, Andreas P.M.
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(2017). Overexpression of the Triose Phosphate Translocator TPT complements the abnormal metabolism and development of plastidial glycolytic glyceraldehyde-3-phosphate dehydrogenase mutants. Plant J 89(6):1146-1158
Flores‐Tornero, María; Anoman, Armand D.; Rosa‐Téllez, Sara; Toujani, Walid; Weber, Andreas P.M.; Eisenhut, Marion; Kurz, Samantha; Alseekh, Saleh; Fernie, Alisdair R.; Muñoz‐Bertomeu, Jesús & Ros, Roc
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(2018). Knock-down of the phosphoserine phosphatase gene effects rather N- than S-metabolism in Arabidopsis thaliana. Front. Plant Sci. 9, 1830
Samuilov, Sladjana; Rademacher, Nadine; Brilhaus, Dominik; Flachbart, Samantha; Arab, Leila; Kopriva, Stanislav; Weber, Andreas P. M.; Mettler-Altmann, Tabea & Rennenberg, Heinz
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(2018). Significance of sulfur assimilation in plant responses to heavy metal stress. Dissertation, Freiburg i.Br.
Samuilov, S
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(2018). The photorespiratory BOU gene mutation alters sulfur metabolism and ist crosstalk with carbon and nitrogen metaboilism in Arabidopsis thaliana. Front. Plant Sci. 9, 1709
Samuilov, Sladjana; Brilhaus, Dominik; Rademacher, Nadine; Flachbart, Samantha; Arab, Leila; Alfarraj, Saleh; Kuhnert, Franziska; Kopriva, Stanislav; Weber, Andreas P. M.; Mettler-Altmann, Tabea & Rennenberg, Heinz
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(2019). Xylem-delivered sulfate tunes ABA biosynthesis in guard cell for stomatal closure. Plant Cell
Batool S, Uslu VV, Rajab H, Geiger D, Malagoli M, Hedrich R, Rennenberg H, Herschbach C, Hell R, Wirtz M
