Höchstauflösendes Fluoreszenzmikroskop

• Subject Area: Basic Research in Biology and Medicine
• Term: Funded in 2014
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 260348792

To identify new strategies for the treatment of disease, advanced imaging strategies with nanoscale resolution are essential to reveal intra- and extracellular processes and the morphology of processes of healthy and diseased cells and tissues in a physiological environment. The currently available optical microscopic techniques and other imaging modalities for basic science are either not applicable to study viable samples or do not offer the resolution for gaining access to the intracellular environment (< 100 nm) of disease processes. We therefore aim to obtain and employ the state-of-the-art "three dimensional stimulated emission depletion" (STED X3) microscopy. STED will enable us to acquire ultra-detailed information on structural and functional processes relevant to disease, in particular cardiovascular pathogenesis. The applicability of STED in live cells or tissues combined with its nanoscale resolution will unravel new mechanisms and hitherto invisible structures in a pathogenic context. This will contribute to a better understanding and treatment of disease.

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: Höchstauflösendes Fluoreszenzmikroskop
• Instrumentation Group: 5090 Spezialmikroskope
• Applicant Institution: Klinikum der Universität München
• Leader: Professor Dr. Christian Weber