Project Details
Projekt
Fate mapping of hematopoietic stem cell activity under steady state and challenges (B11)
Subject Area
Hematology, Oncology
Term
from 2014 to 2022
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 122491522
Hematopoietic stem cells (HSC) are widely studied by transplantation into immune- and blood-cell-depleted recipients. To study hematopoiesis in situ, we developed a mouse model enabling inducible genetic labeling of HSC at different time points of embryonic development and in the adult bone mar-row. Together with mathematical modeling our experiments provide insights into differentiation rates through stem and progenitor populations, residence times and estimates on self-renewal rates in com-partments, and thus a quantitative framework for studying hematopoiesis in situ. We will now investigate the response of the hematopoietic system to perturbations. We will study HSC, marked by Tie2 expression, to refine the kinetic parameters in terms of stem cell proliferation and differentiation decisions towards a better understanding of the complex HSC biology in health and disease.
DFG Programme
Collaborative Research Centres
Applicant Institution
Ruprecht-Karls-Universität Heidelberg
Project Heads
Professor Dr. Thomas Höfer; Professor Dr. Hans-Reimer Rodewald
