Elucidating the function of the deubiquitinating enzyme AMSH in intracellular trafficking in Arabidiopsis thaliana

• Applicant: Professorin Dr. Erika Isono
• Subject Area: Plant Cell and Developmental Biology
• Term: from 2014 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 264673688

Posttranslational modification by ubiquitin is regulating various cellular events and thus controls many aspects of plant development, growth and environmental responses. Therefore, regulation of the ubiquitination status of a given protein by ubiquitinating- as well as deubiquitinating enzymes (DUBs) can play crucial roles for cellular pathways. AMSH proteins are conserved DUBs that are implicated in endocytic protein degradation by interacting with ESCRT-III components, however many of the molecular details of their function and the nature of their target proteins remains yet to be elucidated. We have identified further putative interactors of Arabidopsis AMSH proteins by yeast two-hybrid and immunoprecipitation that are involved in vesicle trafficking, and aim in this project (1) to understand the molecular framework of plant AMSH functions in the intracellular trafficking pathway with regard to its newly identified interactors and (2) to identify the target proteins of AMSH by a two-step ubiquitin-purification method.

• DFG Programme: Research Grants