High-resolution proteomics and functional analyses of the podocyte slit diaphragm and its disease-induced dynamics (A01)

• Subject Area: Nephrology ; Anatomy and Physiology
• Term: from 2015 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 246781735

Podocytes, epithelial pericytes in kidney glomeruli, are key determinants of renal filtration by reconstituting the slit diaphragm (SD), the decisive barrier of the multi-layered filter and target of multiple hereditary diseases. This project directly investigates architecture, organization and function of SD complexes including comprehensive high-resolution proteomic techniques to analyze transmembrane proteins. Moreover we will determine the modification and time-dependent changes of SD complexes by disease-induced stress stimuli.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1140: Kidney Disease - from Genes to Mechanisms (KIDGEM)
• Applicant Institution: Albert-Ludwigs-Universität Freiburg
• Project Heads: Professor Dr. Bernd Fakler; Privatdozent Dr. Florian Grahammer