Structural basis of translation control in eukaryotic 80S ribosomes by phosphorylation of eS6 (A06*)

• Subject Area: Biochemistry
• Term: from 2015 to 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 25065445

As part of the SFB740 we aim to determine the mechanism by which eS6 phosphorylation influences target-mRNA selection of ribosomes. As further reaching hypothesis we will probe if and how translation is governed by eS6-tail modification patterns (or a simple on/off mechanism), i.e. if there is a ribosomal equivalent to CTD control of transcription or the histone code hypothesis. This would highlight a similarity of regulation between translation and transcription and have huge impact on our understanding of translational control. Moreover, with quantitative cross-linking/mass spectrometry (CLMS) and high-resolution CLMS we introduce technologies to protein assemblies that will allow probing a broad range of mechanistic questions in other research projects of this consortium, specifically Project A04, M. Wahl, Project B05, T. Sommer and Project C07, O. Daumke.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 740: From Molecules to Modules: Organisation and Dynamics of Functional Units in Cells
• Applicant Institution: shared FU Berlin and HU Berlin through:; Charité - Universitätsmedizin Berlin
• Co-Applicant Institution: Technische Universität Berlin
• Project Head: Professor Dr. Juri Rappsilber