Establishment of endosomal membrane domains by guanine nucleotide exchange factors (P17)

• Subject Area: Biochemistry ; Plant Physiology
• Term: from 2015 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 180879236

Project P17 investigates how guanine nucleotide exchange factors (GEFs) integrate different cellular cues to properly activate their cognate Rab GTPase in the spatio-temporal coordination of intracellular traffic. The group will elucidate the molecular basis how a particular class of GEFs, LH-GEFs, establish microcompartments by (i) biochemical and structural characterization of regulatory proteins (ii), studying membrane association of LH-GEFs through lipid interactions in vitro and sub-cellular localization in vivo, and (iii) determining the atomic structure and overall architecture of LH-GEFs.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 944: Physiology and Dynamics of Cellular Microcompartments
• Applicant Institution: Universität Osnabrück
• Project Head: Professor Dr. Daniel Kümmel