Broadband microfluidic dielectrometry of biochemical liquids based on microwave precision measurement technique
Biomaterials
Biophysics
Experimental Condensed Matter Physics
Statistical Physics, Nonlinear Dynamics, Complex Systems, Soft and Fluid Matter, Biological Physics
Final Report Abstract
Microwave dielectric spectroscopy is an analysis method for applications in medicine, biological research and food industry. In this project this method has been developed for resonant and non-resonant approaches in the frequency range from 30 GHz to 40 GHz and from 1 GHz to 5 GHz in a temperature range from -30 °C to room temperature (20°C) to analyse if freezing the liquid under test (LuT) will improve the measurement results. For a resonant approach a novel multifrequency whispering gallery mode (WGM) resonator technique and for the non-resonant approach a broadband planar microwave sensor are developed and demonstrated. For both microwave sensors the accuracy has been determined. To do this, for the broadband microwave sensor the SNR of the measurement system was simulated to be SNR = -80 dB + 20*log f/GHz first, then the detection limits has been determined. For the amino acids L-Alanine, L-Arginine, L-Aspargine, Glycine and L-Valine the detection limits for unfrozen and frozen LuT are 200/100, 450/150, 80/10, 450/50, 200/50 with an max. extraction error of 1%. Furthermore, the differentiation of electro-porated and non-electro-porated HeLa cells was possible. For WGM resonator sensor, the permittivity determination accuracy was evaluated as δε⁄ε, where δε represents the most probable errors of the real part of permittivity. The obtained accuracy is equal to 0.4% - 1.2% for different modes while the most probable errors of the imaginary part is equal to 0.3% - 1.1%. We studied the dielectric properties of the basic aliphatic amino acids and polar positive amino acids in solutions of different concentrations using new WGM resonator technique. The technique allows liquid investigation of micro- to nano- liter volumes filled in microfluidic channel on six discrete frequencies in the 30-40 GHz range. The dependencies of the complex permittivity on the molar mass show almost linear behaviour for aliphatic amino acids at different concentrations. The study results are in good agreement with the calculated data obtained by Cole-Cole equation. Moreother our hypothesis of removing screening effect at low temperatures are confirmed by studies of DNA solutions. At -12°C precise measurements of shifts in the resonant frequency and Q-factor changes, simultaneously taken at six frequencies reveal clear investigation of DNA molecules. This approach opens new opportunities for efficient and accurate DNA analysis using microwave sensors in scientific and diagnostic fields.
Publications
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Microwave characterization of aqueous amino acid solutions using the multifrequency WGM resonator technique. Biological Chemistry, 404(2-3), 229-235.
Gubin, Alexey I.; Barannik, Alexander A.; Protsenko, Irina A.; Chekubasheva, Valeriia A.; Lavrinovich, Alexander A.; Cherpak, Nickolay T. & Vitusevich, Svetlana
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Single Whispering-Gallery-Mode Resonator With Microfluidic Chip as a Basis for Multifrequency Microwave Permittivity Measurement of Liquids. IEEE Transactions on Microwave Theory and Techniques, 70(6), 3310-3318.
Gubin, Alexey I.; Protsenko, Irina A.; Barannik, Alexander A.; Cherpak, Nickolay T. & Vitusevich, Svetlana A.
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Multifrequency WGM Resonator Approach for Study of DNA Solutions with Reduced Microwave Screening. 2025 16th German Microwave Conference (GeMiC), 573-576. IEEE.
Chekubasheva, Valeriia A.; Gubin, Alexey I.; Barannik, Alexander A.; Zhulai, Dmytro; Glukhov, Oleg V.; Cherpak, Nickolay T. & Vitusevich, Svetlana
