Molecularly Imprinted Polymers for the selective trageting of phosphotyrosine-containing proteins and peptides

• Antragstellerinnen / Antragsteller: Professorin Dr. Katrin Marcus-Alic; Professor Dr. Börje Sellergren
• Fachliche Zuordnung: Präparative und Physikalische Chemie von Polymeren
• Förderung: Förderung von 2006 bis 2015
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 27215077

In this project, we aim at the realisation of new generations of hierachically imprinted materials based on structural control at both the 1 and 100 nm length scales. Control at the 1 nm length scale is achieved by using host monomers designed to bind to the side chain of given amino acids of modified amino acids, here phosphorylated ones. Control at the 10 nm length scale is achieved by using non-proteinaceous protein surrogates as templates, thus allowing the preparation of size complementary peptide of protein binding sites. We anticipate that combining these approaches will result in robust receptors capable of capturing peptide or protein targets of a certain size but with a defined substitution of substitution pattern. In the area of proteomics, such phases could play the role of class selective affinity phases for fractionation and enrichment of low abundant modified proteins. Phosphorylation is a modification of profound biological importance. Phosphorylated proteins are of critical importance in intercellular signal transduction processes and their analyses forms an important part of proteomics research. The application of MIPs prepared via the above substructure approaches may lead to a new concept in addressing labile biomolecules with MIPs and open the way to a wide range of tailored materials for affinity-based enrichment of proteins, thus representing a major advance in the field of targeted proteomics.

• DFG-Verfahren: Sachbeihilfen