Navigation of osteoblasts in the bone space
Final Report Abstract
Work under DFG grant “Navigation of osteoblasts in the bone space” was driven by the underlying hypothesis that the interplay of biophysical and cellular signals provides coordinates for osteoblast attachment and migration, and thus navigation. Focusing of the osseous surface osteoblasts reside on, the principle discovery of our studies was that the mineralization state of bone independently controls gene expression in osteoblasts. In addition, it was demonstrated for the first time that osteoblastic cells on mineralized and demineralized osseous surfaces favor intracellular protein production and matrix formation, respectively. Above findings were enabled via the combination of a newly devised osseous surface model with cell biology approaches, including global gene expression analysis. To further study the consequence of the gene expression landscape on cellular attachment and migration, fluorescence-labelling means for osteoblasts were systematically explored and ideal labels for future time-lapse microscopy identified.
Publications
- Matrix mineralization controls gene expression in osteoblastic cells. (2018) Exp Cell Res. 372(1):25-34
Wischmann J, Lenze F, Thiel A, Bookbinder S, Querido W, Schmidt O, Burgkart R, von Eisenhart-Rothe R, Richter GHS, Pleshko N, Mayer-Kuckuk P
(See online at https://doi.org/10.1016/j.yexcr.2018.09.005) - Osteoblast migration in vertebrate bone. (2018) Biol Rev Camb Philos Soc. 93(1):350-363
Thiel A, Reumann MK, Boskey A, Wischmann J, von Eisenhart-Rothe R, Mayer-Kuckuk P
(See online at https://doi.org/10.1111/brv.12345)
